Giesco
OPTIMIZATION OF GC-MS AND GC-ECD CHROMATOGRAPHIC METHODS TO DETERMINE THE GREENHOUSE GAS EMISSIONS FROM VINEYARD SOIL OPTIMIZACIÓN DE UN MÉTODO CROMATOGRÁFICO GC-MS Y GC-ECD PARA DETERMINAR LOS GASES DE EFECTO INVERNADERO PROCEDENTES DE LAS EMISIONES DEL SUELO VITÍCOLA
PÉREZ-ÁLVAREZ, Eva P.*; MARTÍNEZ-VIDAURRE, José M; CASTILLO-RIO, Carolina; GARDE-CERDÁN, Teresa Instituto de Ciencias de la Vid y del Vino (Gobierno de La Rioja-CSIC-Universidad de La Rioja). Carretera de Burgos, km. 6. 26007 Logroño, La Rioja, España. * Corresponding author: evapipeal@msn.com
Abstract: Carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4) are the greenhouse gases (GHG) that have suffered a more marked concentration increase since the Industrial Revolution, with agriculture accounting for 13.5% of the worldwide emissions. Of them, N2O is potentially the most harmful to the environment (with a potential for warming 310 times higher than CO2), being its emissions related to the use of nitrogenous and organic amendments in agricultural soils. In order to quantificate the GHG emitted by the soil along the grapevine cycle, a chromatic methodology has been optimized using two detectors, a mass spectrometer (MS) and an electron capture detector (ECD). PVC chambers were placed on soil for sampling the vineyard GHG emissions. Gas samples were collected at time 0, during each hour throughout the morning and 24 hours after being placed on soil. With the purpose to determinate the GHG, a gas chromatographic equipment was provided with two independent columns with two inlets and two detectors (MS and ECD). The GHG chromathographic separation was carried out isothermally (35° C). An automatic injector was employed being He the carrier gas and working in split mode. N2O detection was performed in the ECD, while CH4 and CO2 were detected in the MS detector. Thus, the dynamics of GHG from the vineyard soil using this optimized gas chromatographic method will be studied.
Keywords: GHG, GC-ECD, GC-MS, soil, vineyard
Abstract: Carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4) are the greenhouse gases (GHG) that have suffered a more marked concentration increase since the Industrial Revolution, with agriculture accounting for 13.5% of the worldwide emissions. Of them, N2O is potentially the most harmful to the environment (with a potential for warming 310 times higher than CO2), being its emissions related to the use of nitrogenous and organic amendments in agricultural soils. In order to quantificate the GHG emitted by the soil along the grapevine cycle, a chromatic methodology has been optimized using two detectors, a mass spectrometer (MS) and an electron capture detector (ECD). PVC chambers were placed on soil for sampling the vineyard GHG emissions. Gas samples were collected at time 0, during each hour throughout the morning and 24 hours after being placed on soil. With the purpose to determinate the GHG, a gas chromatographic equipment was provided with two independent columns with two inlets and two detectors (MS and ECD). The GHG chromathographic separation was carried out isothermally (35° C). An automatic injector was employed being He the carrier gas and working in split mode. N2O detection was performed in the ECD, while CH4 and CO2 were detected in the MS detector. Thus, the dynamics of GHG from the vineyard soil using this optimized gas chromatographic method will be studied.
Keywords: GHG, GC-ECD, GC-MS, soil, vineyard