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EFFECT OF PHENYLALANINE AND UREA FOLIAR APPLICATION ON GRAPE AND MUST MICROBIOTA EFECTO DE LA APLICACIÓN FOLIAR DE FENILALANINA Y UREA EN LA MICROBIOTA DE UVAS Y MOSTOS
GONZÁLEZ-ARENZANA, Lucía*; PORTU, Javier; LÓPEZ, Rosa; SANTAMARÍA, Pilar; LÓPEZ-ALFARO, Isabel; GUTIÉRREZ, Ana Rosa ICVV, Instituto de Ciencias de la Vid y el Vino (Universidad de La Rioja, Gobierno de La Rioja, CSIC). Finca La Grajera, Ctra. Burgos, km 6 (LO-20-Salida 13), 26071 Logroño, Spain. *Corresponding author: lucia.gonzalez@unirioja.es
Abstract: Recently, some studies have been conducted to optimize traditional nitrogen supplementation of the soil by promoting the foliar application of different nitrogen sources such as phenylalanine and urea. The aim of this study was to describe the impact of foliar phenylalanine and urea application on grape and must microbial populations. The study was conducted in the experimental Vitis vinifera L.cv. Tempranillo vineyard in La Grajera-ICVV (La Rioja, Spain).The tool used to perform the ecological study was DGGE (Denaturing Gradient Gel Electrophoresis). No significant changes were observed in the microbial communities of grape surface, so the foliar spraying of nitrogen compounds could not affect the natural biofilm of grapes. Nevertheless, some differences were observed in microbial species composition of musts. The phenylalanine must sample was similar to the control, while foliar urea application caused significant changes in microbial diversity and population structure in comparison to the control must.
Keywords: foliar application; grapes; musts; microbiota
Abstract: Recently, some studies have been conducted to optimize traditional nitrogen supplementation of the soil by promoting the foliar application of different nitrogen sources such as phenylalanine and urea. The aim of this study was to describe the impact of foliar phenylalanine and urea application on grape and must microbial populations. The study was conducted in the experimental Vitis vinifera L.cv. Tempranillo vineyard in La Grajera-ICVV (La Rioja, Spain).The tool used to perform the ecological study was DGGE (Denaturing Gradient Gel Electrophoresis). No significant changes were observed in the microbial communities of grape surface, so the foliar spraying of nitrogen compounds could not affect the natural biofilm of grapes. Nevertheless, some differences were observed in microbial species composition of musts. The phenylalanine must sample was similar to the control, while foliar urea application caused significant changes in microbial diversity and population structure in comparison to the control must.
Keywords: foliar application; grapes; musts; microbiota